Anti-NCAPG2 antibody (ab70350)
Key features and details
- Rabbit polyclonal to NCAPG2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NCAPG2 antibody
See all NCAPG2 primary antibodies -
Description
Rabbit polyclonal to NCAPG2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between C terminal residues 1100 -1143 of human NCAPG2. (AAH43404.1)
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Positive control
- WB: HeLa whole cell lysate (ab150035) IP: HeLa whole cell extract
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Relevance
NCAPG2 is 1 of 3 non-SMC subunits that define condensin II. Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that defines condensin II. -
Cellular localization
nucleus -
Database links
- Entrez Gene: 54892 Human
- Omim: 608532 Human
- SwissProt: Q86XI2 Human
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Alternative names
- CAP G2 antibody
- Chromosome associated protein G2 antibody
- Condensin 2 complex subunit G2 antibody
see all
Images
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ab70350 immunoprecipitating NCAPG2 at 6 µg per reaction. Lane 1: ab70350 in HeLa whole cell extract (0.5 or 1 mg per IP reaction; 20% of IP loaded). Lane 2: IgG control. For blotting immunoprecipitated NCAPG2, ab70350 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
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All lanes : Anti-NCAPG2 antibody (ab70350) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 131 kDa
Observed band size: 131 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab70350 has been referenced in 7 publications.
- Dai HT et al. microRNA-375 inhibits the malignant behaviors of hepatic carcinoma cells by targeting NCAPG2. Neoplasma 69:16-27 (2022). PubMed: 34818025
- Jiang S et al. NCAPG2 Maintains Cancer Stemness and Promotes Erlotinib Resistance in Lung Adenocarcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 36139554
- Baergen AK et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
- Deutschman E et al. Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses. J Cell Sci 132:N/A (2019). PubMed: 31653782
- Abramo K et al. A chromosome folding intermediate at the condensin-to-cohesin transition during telophase. Nat Cell Biol 21:1393-1402 (2019). PubMed: 31685986
- Liu W et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB ; Human . PubMed: 20622854
- Centore RC et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell 40:22-33 (2010). WB ; Human . PubMed: 20932472