• Product nameAnti-NCBP1 antibody
    See all NCBP1 primary antibodies
  • Description
    Rabbit polyclonal to NCBP1
  • Specificityab64837 detects endogenous levels of total NCBP1 protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from N-terminal of human NCBP1.

  • Positive control
    • HT29 and LOVO cell extracts.



Our Abpromise guarantee covers the use of ab64837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).


  • FunctionComponent of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.
  • Sequence similaritiesBelongs to the NCBP1 family.
    Contains 1 MIF4G domain.
  • Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • 80 kDa nuclear cap-binding protein antibody
    • CBP80 antibody
    • MGC2087 antibody
    • NCBP 80 kDa subunit antibody
    • NCBP antibody
    • ncbp1 antibody
    • NCBP1_HUMAN antibody
    • nuclear cap binding protein subunit 1 antibody
    • nuclear cap binding protein subunit 1, 80kDa antibody
    • Nuclear cap-binding protein subunit 1 antibody
    • Sto1 antibody
    see all

Anti-NCBP1 antibody images

  • All lanes : Anti-NCBP1 antibody (ab64837) at 1/500 dilution

    Lane 1 : HT29 cell extract
    Lane 2 : LOVO cell extract
    Lane 3 : LOVO cell extract with immunizing peptide

    Predicted band size : 80 kDa
    Observed band size : 80 kDa

References for Anti-NCBP1 antibody (ab64837)

ab64837 has not yet been referenced specifically in any publications.

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