Overview

  • Product nameAnti-NCBP2 antibody [2613C3a]
    See all NCBP2 primary antibodies
  • Description
    Mouse monoclonal [2613C3a] to NCBP2
  • Tested applicationsSuitable for: Dot blot, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: RGDNEEQEKL LKKSCTLYVG NLSFYTTEEQ IYELFSKSGD IKKIIMGLDK MKKTACGFCF VEYYSRADAE NAMRYINGTR LDDRIIRTDW DAGFKEGRQY GRGRSGGQV, corresponding to internal sequence amino acids 26-134 of Human NCBP2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab84854 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
  • ClonalityMonoclonal
  • Clone number2613C3a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.

Target

  • RelevanceNCBP2 is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. It has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • 20 kDa nuclear cap binding protein antibody
    • CBC2 antibody
    • CBP20 antibody
    • Cell proliferation inducing gene 55 protein antibody
    • NCBP 2 antibody
    • NCBP 20 kDa subunit antibody
    • NCBP interacting protein 1 antibody
    • NIP1 antibody
    • Nuclear cap binding protein subunit 2 antibody
    • PIG55 antibody
    see all

Anti-NCBP2 antibody [2613C3a] images

  • Anti-NCBP2 antibody [2613C3a] (ab84854) + Immunogen (Recombinant Human NCBP2 fragment)

    Predicted band size : 18 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab84854 staining NCBP2 in HeLa cells by Immunofluorescence. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS.

References for Anti-NCBP2 antibody [2613C3a] (ab84854)

ab84854 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"