Overview

  • Product nameAnti-NCBP2 antibody
    See all NCBP2 primary antibodies
  • Description
    Rabbit polyclonal to NCBP2
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 106-156 (LDDRIIRTDW DAGFKEGRQY GRGRSGGQVR DEYRQDYDAG RGGYGKLAQN Q) of Human NCBP2 (NP_031388.2).

  • Positive control
    • HeLa and Mouse NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 18 kDa.
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceNCBP2 is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. It has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • 20 kDa nuclear cap binding protein antibody
    • CBC2 antibody
    • CBP20 antibody
    • Cell proliferation inducing gene 55 protein antibody
    • NCBP 2 antibody
    • NCBP 20 kDa subunit antibody
    • NCBP interacting protein 1 antibody
    • NIP1 antibody
    • Nuclear cap binding protein subunit 2 antibody
    • PIG55 antibody
    see all

Anti-NCBP2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling NCBP2 with ab91560 at 1/5000 (0.2µg/ml) and 1/1000 (1 µg/ml). Detection: DAB.
  • All lanes : Anti-NCBP2 antibody (ab91560) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 18 kDa


    Exposure time : 3 minutes
  • ab91560 at 1 µg/ml detecting NCBP2 in HeLa whole cell lysate. Lane 1; IP with ab91560 at 10 µg/mg of lysate. Lane 2; IP with an antibody which recognizes an upstream epitope of NCBP2. Lane 3; Control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection utilised ECL with a 30 second exposure.

References for Anti-NCBP2 antibody (ab91560)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"