1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RelevanceNCBP2 is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. It has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling NCBP2 with ab91560 at 1/5000 (0.2µg/ml) and 1/1000 (1 µg/ml). Detection: DAB.
Western blot - NCBP2 antibody (ab91560)
All lanes : Anti-NCBP2 antibody (ab91560) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : Mouse NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 18 kDa
Exposure time : 3 minutes
Immunoprecipitation - NCBP2 antibody (ab91560)
ab91560 at 1 µg/ml detecting NCBP2 in HeLa whole cell lysate. Lane 1; IP with ab91560 at 10 µg/mg of lysate. Lane 2; IP with an antibody which recognizes an upstream epitope of NCBP2. Lane 3; Control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection utilised ECL with a 30 second exposure.