Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab15707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 (PMID 19250909).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
    • Tissue specificity
      Widely expressed (at protein level).
    • Pathway
      Protein modification; protein neddylation.
    • Sequence similarities
      Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
    • Information by UniProt
    • Database links
    • Alternative names
      • NCE2 antibody
      • NEDD8 carrier protein UBE2F antibody
      • NEDD8 conjugating enzyme antibody
      • NEDD8 conjugating enzyme UBE2F antibody
      • NEDD8 protein ligase UBE2F antibody
      • NEDD8-conjugating enzyme 2 antibody
      • NEDD8-conjugating enzyme UBE2F antibody
      • ube2f antibody
      • UBE2F_HUMAN antibody
      • Ubiquitin conjugating enzyme E2F antibody
      • Ubiquitin conjugating enzyme E2F (putative) antibody
      • Ubiquitin-conjugating enzyme E2 F antibody
      see all

    Images

    • All lanes : Anti-NCE2 antibody (ab15707) at 1/1000 dilution

      Lane 1 : Endogenous Nce2 from mouse 3T3 cells
      Lane 2 : NIH 3T3 cells infected with ShRNA of mouse Nce2
      Lane 3 : NIH3T3 cells infected with ShRNA of Nce2 and MCSV-NCE2his flag-IRES-GFP (human NCE2)

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated rabbit polyclonal to goat IgG
      Developed using the ECL technique

      Exposure time : 10 minutes

      See Abreview

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    35 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Sample
    Mouse Cell lysate - whole cell (HEK293, HCT116, U2OS and Mouse Embryonic Fibroblas)
    Specification
    HEK293, HCT116, U2OS and Mouse Embryonic Fibroblas
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 18 2013

    Application
    Western blot
    Loading amount
    35 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Sample
    Human Cell lysate - whole cell (hEK293, HCT116, U2OS and Mouse Embryonic Fibroblas)
    Specification
    hEK293, HCT116, U2OS and Mouse Embryonic Fibroblas
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 18 2013

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human cell lines HeLa, Jurkat, U2OS, 293T and IRM9)
    Loading amount
    20 µg
    Specification
    Human cell lines HeLa, Jurkat, U2OS, 293T and IRM9
    Gel Running Conditions
    Reduced Denaturing (10% and 4-12%)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: +4°C
    Username

    Dr. Martine Roussel

    Verified customer

    Submitted Apr 28 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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