The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 (PMID 19250909).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Widely expressed (at protein level).
Protein modification; protein neddylation.
Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.