The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Tissue specificityWidely expressed (at protein level).
PathwayProtein modification; protein neddylation.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.