Overview

  • Product name
  • Description
    Goat polyclonal to NCF1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Guinea pig, Cow, Dog, Human, Pig, Chinese hamster
  • Immunogen

    Synthetic peptide with a Cysteine residue linker, corresponding to internal sequence amino acids 217-230 of Mouse NCF1 (NP_035006.3).

  • Positive control
    • Mouse Thymus lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab166930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 45 kDa.

Target

  • Function
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • Involvement in disease
    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similarities
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

Images

  • Anti-NCF1 antibody (ab166930) at 0.03 µg/ml + Mouse Thymus lysates at 35 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa

References

ab166930 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
60 µg
Treatment
TNF-a 30min
Specification
heart
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Baptiste Jude

Verified customer

Submitted Jul 07 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (lung)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
70 µg
Treatment
intermittent hypoxia
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 31 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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