Overview

  • Product name
  • Description
    Rabbit polyclonal to NCF1
  • Specificity
    This antibody detects endogenous levels of total Neutrophil Cytosol Factor 1 protein
  • Tested applications
    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human NCF1 around the phosphorylation site of serine 304 (R-S-SP-I-R)

  • Positive control
    • UV treated COS7 cell extracts; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab63361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • Involvement in disease
    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similarities
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

Images

  • All lanes : Anti-NCF1 antibody (ab63361) at 1/500 dilution

    Lane 1 : extracts from COS7 cells, treated with UV (15mins)
    Lane 2 : extracts from COS7 cells, treated with UV (15mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 70 kDa (possible non-specific binding),84 kDa (possible non-specific binding).
  • Immunofluorescence analysis of NCF1 expression in HeLa cells, using 1/500 ab63361. Left: untreated cells. Right: cells treated with immunising peptide.

References

ab63361 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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