Overview

  • Product name
  • Description
    Goat polyclonal to NCF1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Pig
  • Immunogen

    Synthetic peptide:

    CSESTKRKLASAV

    , corresponding to C terminal amino acids 378-390 of Human NCF1.

  • Positive control
    • U937 cell lysates
  • General notes
    Principal Names - NCF1; p47phox; Neutrophil cytosolic factor-1 (47 kDa, chronic granulomatous disease, autosomal 1) Official Gene Symbol - NCF1 GenBank Accession Number – NP_000256 LocusLink ID - 4687


    NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.

Properties

Applications

Our Abpromise guarantee covers the use of ab795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human NCF1 peptide (ab22781).

Target

  • Function
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • Involvement in disease
    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similarities
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

Images

  • Anti-NCF1 antibody (ab795) at 1/0.5 dilution + U937 lysate in RIPA buffer. at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • Anti-NCF1 antibody (ab795) at 1.5 µg/ml + Recombinant Human NCF1 protein (ab98054) at 0.1 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References

This product has been referenced in:
  • Lu XY  et al. Acute ethanol induces apoptosis by stimulating TRPC6 via elevation of superoxide in oxygenated podocytes. Biochim Biophys Acta 1853:965-74 (2015). Read more (PubMed: 25601712) »
  • Jha P  et al. Genome-wide methylation profiling identifies an essential role of reactive oxygen species in pediatric glioblastoma multiforme and validates a methylome specific for H3 histone family 3A with absence of G-CIMP/isocitrate dehydrogenase 1 mutation. Neuro Oncol N/A:N/A (2014). Human . Read more (PubMed: 24997139) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 01 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 01 2016

Thank you for contacting us.

Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

The antibodies we have are; ab74095, ab63331, ab11...

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Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: http://www.abcam.com/index.html?datasheet=109366). Click here (or use the...

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Thank you very much for your interest in ab795. To our knowledge, ab795 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab795 now, test it in rat and submit feedback to us in the form of an Abreview. I...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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