Overview

  • Product nameAnti-NCF1 (phospho S304) antibody
    See all NCF1 primary antibodies
  • Description
    Rabbit polyclonal to NCF1 (phospho S304)
  • SpecificityDetects NCF1 only when phosphorylated at serine 304.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human NCF1 around the phosphorylation site of serine 304 (R-S-SP-I-R).

  • Positive control
    • COS7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab63554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ELISA 1/15000.

Target

  • FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • Involvement in diseaseDefects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similaritiesContains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

Anti-NCF1 (phospho S304) antibody images

  • All lanes : Anti-NCF1 (phospho S304) antibody (ab63554) at 1/500 dilution

    Lane 1 : extracts from COS7 cells treated with UV (15mins)
    Lane 2 : A549 cells extracts
    Lane 3 : A549 cells extracts with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 45 kDa

References for Anti-NCF1 (phospho S304) antibody (ab63554)

This product has been referenced in:
  • Leverence JT  et al. Lipopolysaccharide-induced cytokine expression in alveolar epithelial cells: Role of PKC?-mediated p47phox phosphorylation. Chem Biol Interact : (2010). WB ; Human . Read more (PubMed: 20920494) »

See 1 Publication for this product

Product Wall

DISCOUNT CODE: XXXXXXX
Expiration date: 31-05-2013
Value: £235

I am very pleased to hear you would like to accept our offer and test ab63554 in Flow Cytometry. This code will give you ab63554 off your next order before the expiration d...

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Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

The antibodies we have are; ab74095, ab63331, ab11...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"