• Product name
    Anti-NCF4 antibody [EP2142Y]
    See all NCF4 primary antibodies
  • Description
    Rabbit monoclonal [EP2142Y] to NCF4
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human NCF4

  • Positive control
    • HL60 cell lysate; human spleen tissue. IF/ICC: MCF7 cell line.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/25000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IP 1/50.
IHC-P 1/100 - 1/250.
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Function
    Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
  • Tissue specificity
    Expression is restricted to hematopoietic cells.
  • Sequence similarities
    Contains 1 PX (phox homology) domain.
    Contains 1 SH3 domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CGD3 antibody
    • MGC3810 antibody
    • NCF 4 antibody
    • NCF antibody
    • NCF-4 antibody
    • Ncf4 antibody
    • NCF4_HUMAN antibody
    • Neutrophil cytosol factor 4 antibody
    • Neutrophil cytosolic factor 4 antibody
    • Neutrophil NADPH oxidase factor 4 antibody
    • p40-phox antibody
    • p40phox antibody
    • SH3 and PX domain-containing protein 4 antibody
    • SH3PXD4 antibody
    see all

Anti-NCF4 antibody [EP2142Y] images

  • ICC/IF image of ab76158 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76158, 1/25 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab76158 at 1/100-1/250 dilution staining NCF4 in human spleen by Immunohistochemistry, Paraffin-embedded tissue.
  • Anti-NCF4 antibody [EP2142Y] (ab76158) at 1/50000 dilution + HL60 cell lysate at 10 µg

    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Overlay histogram showing K562 cells stained with ab76158 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76158, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-NCF4 antibody [EP2142Y] (ab76158)

This product has been referenced in:
  • Merling RK  et al. An AAVS1-targeted minigene platform for correction of iPSCs from all five types of chronic granulomatous disease. Mol Ther 23:147-57 (2015). Read more (PubMed: 25288370) »
  • Lamb FS  et al. Endotoxin priming of neutrophils requires endocytosis and NADPH oxidase-dependent endosomal reactive oxygen species. J Biol Chem 287:12395-404 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22235113) »

See all 4 Publications for this product

Product Wall

Western blot
Human Cell lysate - whole cell (monocyte-derived macrophage)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
monocyte-derived macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted May 25 2015


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