• Product name
    Anti-Nck 1/2 antibody [AF6D7]
    See all Nck 1/2 primary antibodies
  • Description
    Mouse monoclonal [AF6D7] to Nck 1/2
  • Specificity
    ab80620 is specific for both Nck 1 and Nck 2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    His-tagged recombinant full length Human NCK2, purified from E.coli

  • Positive control
    • Lysates from HeLa, U937, A431, SK-N-MC, Ramos or PC12 cells.



Our Abpromise guarantee covers the use of ab80620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use 0.5µl.
    WB: 1/1000. Predicted molecular weight: 47 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      NCK1 is one of the adaptor proteins which mediate specific protein-protein interactions in signaling processes. Adaptor proteins usually contain several domains like SH2 and SH3 which allow specific interactions with other specific proteins. NCK1 and NCK2 show high sequence identity (68%) and have three SH3 domains and a C-terminal SH2 domain. Both of them bind receptor tyrosine kinases such as PDGFR and other tyrosine phosphorylated proteins via their SH2 domains. Various molecules which interact with SH domains of Nck and regulate signaling process of actin cytoskeleton reorganization have been identified. Ncks are thought to have important functions in the development of mesodermal structures during embryogenesis, linked to a role in cell movement and cytoskeletal reorganization. Ncks also have a function in modulating mRNA translation at the level of initiation by interacting eukayotic initiation factor 2 (eIF2). Under stressed conditions, protein synthesis is reduced by inhibiting the activity of eIF2 through phosphorylation, transiently inhibiting recycling of eIF2 into its active form.
    • Cellular localization
      Cytoplasmic and Endoplasmic reticulum
    • Database links
    • Alternative names
      • GRB4 antibody
      • melanoma NCK protein antibody
      • MGC12668 antibody
      • NCK antibody
      • NCK adaptor protein 1 antibody
      • NCK adaptor protein 2 antibody
      • NCK tyrosine kinase antibody
      • NCKalpha antibody
      • NCKbeta antibody
      • non catalytic region of tyrosine kinase antibody
      • noncatalytic region of tyrosine kinase, beta antibody
      • SH2/SH3 adaptor protein NCK alpha antibody
      • SH2/SH3 adaptor protein NCK beta antibody
      see all


    • All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/2000 dilution

      Lane 1 : HeLa Cell Lysate
      Lane 2 : 239T Cell Lysate
      Lane 3 : PC12 Cell Lysate
      Lane 4 : NIH3T3 Cell Lysate

      Predicted band size : 47 kDa

      Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

    • Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

    • All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/5000 dilution

      Lane 1 : NCK1 Recombinant Protein
      Lane 2 : NCK2 Recombinant Protein

      Predicted band size : 47 kDa

      Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

    • All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : SK-N-MC cell lysate
      Lane 5 : Ramos cell lysate
      Lane 6 : PC12 cell lysate

      Predicted band size : 47 kDa


    ab80620 has not yet been referenced specifically in any publications.

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