Overview

  • Product name
    Anti-Nck antibody [11D10]
    See all Nck primary antibodies
  • Description
    Mouse monoclonal [11D10] to Nck
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    His-tagged recombinant full length Human protein, purified from E. Coli

  • Positive control
    • WB: HeLa, RD, PC12 and NIH3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab77124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Post-translational
    modifications
    Phosphorylated on Ser and Tyr residues.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic protein NCK1 antibody
    • Melanoma Nck protein antibody
    • MGC12668 antibody
    • NCK 1 antibody
    • NCK adaptor protein 1 antibody
    • NCK alpha antibody
    • NCK antibody
    • NCK tyrosine kinase antibody
    • Nck-1 antibody
    • NCK1 antibody
    • NCK1_HUMAN antibody
    • NCKalpha antibody
    • Non catalytic region of tyrosine kinase antibody
    • SH2/SH3 adaptor protein NCK alpha antibody
    • SH2/SH3 adaptor protein NCK-alpha antibody
    see all

Images

  • All lanes : Anti-Nck antibody [11D10] (ab77124) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : RD cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH3T3 cell lysate

    Predicted band size: 47 kDa



    Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.

References

ab77124 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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