Overview

  • Product name
  • Description
    Mouse polyclonal to Nck
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Full length Human Nck (amino acids 1-377; NP_006144.1).

  • Positive control
    • Nck transfected 293T cell lysate; A431 cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Post-translational
    modifications
    Phosphorylated on Ser and Tyr residues.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic protein NCK1 antibody
    • Melanoma Nck protein antibody
    • MGC12668 antibody
    • NCK 1 antibody
    • NCK adaptor protein 1 antibody
    • NCK alpha antibody
    • NCK antibody
    • NCK tyrosine kinase antibody
    • Nck-1 antibody
    • NCK1 antibody
    • NCK1_HUMAN antibody
    • NCKalpha antibody
    • Non catalytic region of tyrosine kinase antibody
    • SH2/SH3 adaptor protein NCK alpha antibody
    • SH2/SH3 adaptor protein NCK-alpha antibody
    see all

Images

  • Anti-Nck antibody (ab168940) at 1 µg/ml + A431 cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 43 kDa

  • All lanes : Anti-Nck antibody (ab168940) at 1 µg/ml

    Lane 1 : Nck transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 43 kDa

  • Immunofluorescent analysis of HeLa cells labeling Nck with ab168940 at 20 µg/ml.

References

This product has been referenced in:
  • Dubrac A  et al. Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization. Circulation 133:409-21 (2016). Read more (PubMed: 26659946) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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