Overview

  • Product nameAnti-Nck beta antibody
    See all Nck beta primary antibodies
  • Description
    Rabbit polyclonal to Nck beta
  • Tested applicationsSuitable for: WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    EAPSFLSLRRGTALSN

    conjugated to KLH, corresponding to amino acids 175-190 of Mouse Nck beta.

  • General notesBefore the addition of glycerol this product is at 1mg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab14590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 19948823

Target

  • FunctionAdapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Cellular localizationCytoplasm. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic protein NCK2 antibody
    • GRB4 antibody
    • Growth factor receptor bound protein 4 antibody
    • NCK adaptor protein 2 antibody
    • Nck-2 antibody
    • NCK2 antibody
    • NCK2_HUMAN antibody
    • NCKbeta antibody
    • Non-catalytic region of tyrosine kinase adaptor protein 2 antibody
    • Noncatalytic region of tyrosine kinase beta antibody
    • SH2/SH3 adaptor protein NCK-beta antibody
    see all

References for Anti-Nck beta antibody (ab14590)

This product has been referenced in:
  • Clouthier DL  et al. Requisite role for Nck adaptors in cardiovascular development, endothelial-to-mesenchymal transition, and directed cell migration. Mol Cell Biol 35:1573-87 (2015). ICC/IF . Read more (PubMed: 25691664) »
  • Shi D  et al. Association of HK2 and NCK2 with normal tension glaucoma in the Japanese population. PLoS One 8:e54115 (2013). IHC (PFA fixed) ; Mouse . Read more (PubMed: 23349798) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"