Anti-Nck beta antibody [AF3F11] (ab80618)

Overview

  • Product name
    Anti-Nck beta antibody [AF3F11]
    See all Nck beta primary antibodies
  • Description
    Mouse monoclonal [AF3F11] to Nck beta
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    His-tagged recombinant full length Human protein, expressed in E. Coli

  • Positive control
    • A431 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP Use at an assay dependent dilution.

Target

  • Function
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic protein NCK2 antibody
    • GRB4 antibody
    • Growth factor receptor bound protein 4 antibody
    • NCK adaptor protein 2 antibody
    • Nck-2 antibody
    • NCK2 antibody
    • NCK2_HUMAN antibody
    • NCKbeta antibody
    • Non-catalytic region of tyrosine kinase adaptor protein 2 antibody
    • Noncatalytic region of tyrosine kinase beta antibody
    • SH2/SH3 adaptor protein NCK-beta antibody
    see all

Images

  • All lanes : Anti-Nck beta antibody [AF3F11] (ab80618) at 1/2000 dilution

    Lane 1 : RD Cell lysate
    Lane 2 : 293T Cell lysate
    Lane 3 : PC12 Cell lysate
    Lane 4 : NIH3T3 Cell lysate


    Predicted band size : 43 kDa

    Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

  • All lanes : Anti-Nck beta antibody [AF3F11] (ab80618) at 1/2000 dilution

    Lane 1 : NCK 1
    Lane 2 : NCK beta


    Predicted band size : 43 kDa

    Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

  • All lanes : Anti-Nck beta antibody [AF3F11] (ab80618) at 1/2000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : Hepg2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : SK-N-MC cell lysate


    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References

ab80618 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry regarding ab80618.

I can confirm that the cross-reactivity of this antibody has been tested in Rat and Human samples. Other species has not been determined yet. For further information, please could you advise your ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up