Overview

  • Product name
    Anti-Nck beta antibody [EPR3333]
    See all Nck beta primary antibodies
  • Description
    Rabbit monoclonal [EPR3333] to Nck beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NCK beta

  • Positive control
    • Human spleen lysate, human fetal brain lysate, SH-SY5Y lysate, Human fetal kidney lysate; Human breast ductal carcinoma tissue; HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

  • Function
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic protein NCK2 antibody
    • GRB4 antibody
    • Growth factor receptor bound protein 4 antibody
    • NCK adaptor protein 2 antibody
    • Nck-2 antibody
    • NCK2 antibody
    • NCK2_HUMAN antibody
    • NCKbeta antibody
    • Non-catalytic region of tyrosine kinase adaptor protein 2 antibody
    • Noncatalytic region of tyrosine kinase beta antibody
    • SH2/SH3 adaptor protein NCK-beta antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue using ab109239 at a dilution of 1/50
  • Immunofluorescent staining of HeLa cells using ab109239 at a dilution of 1/100
  • All lanes : Anti-Nck beta antibody [EPR3333] (ab109239) at 1/1000 dilution

    Lane 1 : Human spleen lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : SH-SY5Y lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 43 kDa

References

This product has been referenced in:
  • Dubrac A  et al. Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization. Circulation 133:409-21 (2016). Read more (PubMed: 26659946) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109239.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up