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Product name Anti-Nck beta antibody [EPR3333] See all Nck beta primary antibodies
Rabbit monoclonal [EPR3333] to Nck beta
Tested applications Suitable for:
WB, IHC-P, ICC/IF more details
Mouse, Rat, Human
A synthetic peptide corresponding to residues in Human NCK beta
Human spleen lysate, human fetal brain lysate, SH-SY5Y lysate, Human fetal kidney lysate; Human breast ductal carcinoma tissue; HeLa cells
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR3333
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/100 - 1/250.
Function Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Tissue specificity Ubiquitous.
Sequence similarities Contains 1 SH2 domain. Contains 3 SH3 domains.
Cellular localization Cytoplasm. Endoplasmic reticulum.
Information by UniProt
Cytoplasmic protein NCK2 antibody
Growth factor receptor bound protein 4 antibody
Anti-Nck beta antibody [EPR3333] images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nck beta antibody [EPR3333] (ab109239)
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue using ab109239 at a dilution of 1/50
Immunocytochemistry/ Immunofluorescence - Nck beta antibody [EPR3333] (ab109239)
Immunofluorescent staining of HeLa cells using ab109239 at a dilution of 1/100
Western blot - Nck beta antibody [EPR3333] (ab109239)
All lanes : Anti-Nck beta antibody [EPR3333] (ab109239) at 1/1000 dilution Lane 1 : Human spleen lysate Lane 2 : Human fetal brain lysate Lane 3 : SH-SY5Y lysate Lane 4 : Human fetal kidney lysate Lysates/proteins at 10 µg per lane. Predicted band size : 43 kDa
References for Anti-Nck beta antibody [EPR3333] (ab109239)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"