Anti-NCKAP1 antibody (ab126061)
Key features and details
- Rabbit polyclonal to NCKAP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NCKAP1 antibody
See all NCKAP1 primary antibodies -
Description
Rabbit polyclonal to NCKAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 2-298 of Human NCKAP1 (Q9Y2A7).
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Positive control
- NT2D1, IMR32, U-87 MG, MCF7 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 128 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 128 kDa. |
Target
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Function
Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. -
Tissue specificity
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. -
Sequence similarities
Belongs to the HEM-1/HEM-2 family. -
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. At the interface between the lamellipodial actin meshwork and the membrane. - Information by UniProt
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Database links
- Entrez Gene: 10787 Human
- Entrez Gene: 50884 Mouse
- Entrez Gene: 58823 Rat
- Entrez Gene: 494653 Xenopus laevis
- Omim: 604891 Human
- SwissProt: Q9Y2A7 Human
- SwissProt: P28660 Mouse
- SwissProt: P55161 Rat
see all -
Alternative names
- FLJ11291 antibody
- KIAA0587 antibody
- Membrane associated protein HEM2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126061 has been referenced in 1 publication.
- Whitelaw JA et al. The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Cells 9:N/A (2020). PubMed: 32646006