Overview

  • Product nameAnti-NCKIPSD antibody
    See all NCKIPSD primary antibodies
  • Description
    Mouse polyclonal to NCKIPSD
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length human NCKIPSD protein (NP_909119.1).

  • Positive control
    • Rat brain tissue lysate, HeLa cell lysate and NCKIPSD transfected 293T cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23024796

Target

  • FunctionHas an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.
  • Tissue specificityHighest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
  • Involvement in diseaseNote=A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with MLL.
  • Sequence similaritiesContains 1 SH3 domain.
  • Cellular localizationNucleus. Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 54 kDa VacA-interacting protein antibody
    • 54 kDa vimentin-interacting protein antibody
    • 90 kDa SH3 protein interacting with Nck antibody
    • AF3p21 antibody
    • dia interacting protein antibody
    • Dia-interacting protein 1 antibody
    • Diaphanous protein-interacting protein antibody
    • DIP antibody
    • DIP-1 antibody
    • DIP1 antibody
    • NCK interacting protein with SH3 domain antibody
    • NCK-interacting protein with SH3 domain antibody
    • Nckipsd antibody
    • ORF1 antibody
    • SH3 adapter protein SPIN90 antibody
    • SH3 protein interacting with Nck 90 kDa antibody
    • SPIN90 antibody
    • SPN90_HUMAN antibody
    • VIP54 antibody
    • WASLBP antibody
    • WASP-interacting SH3-domain protein antibody
    • WISH antibody
    • wiskott- Aldrich syndrome protein-interacting protein antibody
    • Wiskott-Aldrich syndrome protein-interacting protein antibody
    see all

Anti-NCKIPSD antibody images

  • Anti-NCKIPSD antibody (ab88467) at 1 µg/ml + rat brain tissue lysate at 50 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • Anti-NCKIPSD antibody (ab88467) at 1 µg/ml + HeLa cell lysate at 50 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • All lanes : Anti-NCKIPSD antibody (ab88467) at 1 µg/ml

    Lane 1 : NCKIPSD transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa

References for Anti-NCKIPSD antibody (ab88467)

This product has been referenced in:
  • Wyse MM  et al. Dia-Interacting Protein (DIP) Imposes Migratory Plasticity in mDia2-Dependent Tumor Cells in Three-Dimensional Matrices. PLoS One 7:e45085 (2012). ICC/IF . Read more (PubMed: 23024796) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.01%TritonX-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Ben Siemsen

Verified customer

Submitted Jun 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"