SpecificityDetects rat sodium / calcium - potassium exchanger (NCKX 2) overexpressed in HEK cells transfected with the rat gene. By Western blot, the NCX2 antibody detects the recombinant rat protein (78kDa). Immunohistochemical staining of rat NCX2 in HEK cells transfected with the rat gene results in staining of the plasma membrane.
FunctionCritical component of the visual transduction cascade, controlling the calcium concentration of outer segments during light and darkness. Light causes a rapid lowering of cytosolic free calcium in the outer segment of both retinal rod and cone photoreceptors and the light-induced lowering of calcium is caused by extrusion via this protein which plays a key role in the process of light adaptation. Transports 1 Ca(2+) and 1 K(+) in exchange for 4 Na(+).
Sequence similaritiesBelongs to the sodium/potassium/calcium exchanger family. SLC24A subfamily.