Recombinant
RabMAb

Anti-NCLN antibody [EPR8529] (ab170100)

Overview

  • Product name
    Anti-NCLN antibody [EPR8529]
    See all NCLN primary antibodies
  • Description
    Rabbit monoclonal [EPR8529] to NCLN
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human NCLN (UniProt ID: Q969V3)

  • Positive control
    • A431, HeLa or 293T lysate, Human pancreas or uterus tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab170100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    Images

    • All lanes : Anti-NCLN antibody [EPR8529] (ab170100) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
    • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling NCLN with ab170100 at a 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling NCLN with ab170100 at a 1/100 dilution.

    References

    ab170100 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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