• Product name
  • Description
    Rabbit polyclonal to NCOA4
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 564 and 614 of Human NCOA4 (NP_005428.1).

  • Positive control
    • HeLa and 293T whole cell lysates.



Our Abpromise guarantee covers the use of ab86707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
IP Use at 10 µg/mg of lysate.


  • Function
    Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
  • Tissue specificity
    Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis.
  • Involvement in disease
    Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa androgen receptor activator antibody
    • 70 kDa androgen receptor coactivator antibody
    • 70 kDa AR activator antibody
    • 70 kDa AR-activator antibody
    • Androgen receptor coactivator 70 kD antibody
    • Androgen receptor coactivator 70 kDa protein antibody
    • Androgen receptor-associated protein of 70 kDa antibody
    • ARA 70 antibody
    • ARA70 antibody
    • DKFZp762E1112 antibody
    • ELE 1 antibody
    • ELE1 antibody
    • ELE1/ret TK antibody
    • NCOA 4 antibody
    • NCoA-4 antibody
    • NCOA4 antibody
    • NCOA4_HUMAN antibody
    • Nuclear receptor coactivator 4 antibody
    • Papillary thyroid carcinoma 3 antibody
    • PTC 3 antibody
    • PTC3 antibody
    • RET activating gene ELE1 antibody
    • Ret activating protein ELE1 antibody
    • Ret-activating protein ELE1 antibody
    • RFG antibody
    see all


  • All lanes : Anti-NCOA4 antibody (ab86707) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 70 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 171 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

    Detection: Chemiluminescence with an exposure time of 3 minutes.
  • Western Blot detection of NCOA4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86707 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.


ab86707 has not yet been referenced specifically in any publications.

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