• Product nameAnti-NCOA4 antibody
    See all NCOA4 primary antibodies
  • Description
    Mouse monoclonal to NCOA4
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the C terminal amino acids 505-614 (EGSPKEVPGT EDRAGKQKFK SPMNTSWCSF NTADWVLPGK KMGNLSQLSS GEDKWLLRKK AQEVLLNSPL QEEHNFPPDH YGLPAVCDLF ACMQLKVDKE KWLYRTPLQM) of human NCOA4 (NP_005428) linked to a proprietary tag.

  • Positive control
    • IHC-P: Human pancreas WB: The immunogen; HeLa nuclear extract



Our Abpromise guarantee covers the use of ab89222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 70 kDa.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.


  • FunctionEnhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
  • Tissue specificityWidely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis.
  • Involvement in diseaseDefects in NCOA4 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa androgen receptor activator antibody
    • 70 kDa androgen receptor coactivator antibody
    • 70 kDa AR activator antibody
    • 70 kDa AR-activator antibody
    • Androgen receptor coactivator 70 kD antibody
    • Androgen receptor coactivator 70 kDa protein antibody
    • Androgen receptor-associated protein of 70 kDa antibody
    • ARA 70 antibody
    • ARA70 antibody
    • DKFZp762E1112 antibody
    • ELE 1 antibody
    • ELE1 antibody
    • ELE1/ret TK antibody
    • NCOA 4 antibody
    • NCoA-4 antibody
    • NCOA4 antibody
    • NCOA4_HUMAN antibody
    • Nuclear receptor coactivator 4 antibody
    • Papillary thyroid carcinoma 3 antibody
    • PTC 3 antibody
    • PTC3 antibody
    • RET activating gene ELE1 antibody
    • Ret activating protein ELE1 antibody
    • Ret-activating protein ELE1 antibody
    • RFG antibody
    see all

Anti-NCOA4 antibody images

  • Anti-NCOA4 antibody (ab89222) at 5 µg/ml + immunogen at 0.2 µg
    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-NCOA4 antibody (ab89222) at 5 µg/ml + HeLa nuclear extract at 50 µg
    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : ~60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~13 kDa. We are unsure as to the identity of these extra bands.
  • ab89222, at 3 µg/ml, staining NCOA4 in formalin fixed, paraffin embedded human pancreas tissue by Immunoperoxidase /Immunohistochemistry.
  • ab89222, at 10 µg/ml, detecting recombinant tagged NCOA4 in a sandwich ELISA. The detection limit for recombinant tagged NCOA4 is approximately 0.3 ng/ml when using ab89222 as a capture antibody.

References for Anti-NCOA4 antibody (ab89222)

ab89222 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab89222.
Please use the links above to contact us or submit feedback about this product.