Overview

  • Product name
  • Description
    Rabbit polyclonal to NCOA62
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 239-516 of Human NCOA62.

  • Positive control
    • A431, H1299, HeLa, HepG2, Molt-4, Raji and Mouse liver cell lysates; Cal27 xenograft; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    Nuclear receptor coactivator NCoA62 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Bx 42 antibody
    • Bx42 antibody
    • Homolog of Drosophila BX42 antibody
    • MGC119379 antibody
    • NCOA 62 antibody
    • Nuclear protein SkiP antibody
    • Nuclear receptor coactivator 62 kD antibody
    • Nuclear receptor coactivator NCoA 62 antibody
    • Nuclear receptor coactivator NCoA62 antibody
    • Prp 45 antibody
    • Prp45 antibody
    • PRPF 45 antibody
    • PRPF45 antibody
    • Ski interacting protein antibody
    • SKIIP antibody
    • SKIP antibody
    • SNW 1 antibody
    • SNW domain containing 1 antibody
    • SNW domain containing protein 1 antibody
    • SNW1 antibody
    • SNW1 protein antibody
    see all

Images

  • Anti-NCOA62 antibody (ab153887) at 1/10000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Anti-NCOA62 antibody (ab153887) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling NCOA62 with ab153887 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labeling NCOA62 with ab153887 at 1/100 dilution.

References

This product has been referenced in:
  • Bercht Pfleghaar K  et al. Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells. Nucleus 6:66-76 (2015). Read more (PubMed: 25738644) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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