Overview

  • Product nameAnti-NCOA62 antibodySee all NCOA62 primary antibodies ...
  • Description
    Rabbit polyclonal to NCOA62
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 112 - 166 of Human NCOA62.

  • Positive control
    • Jurkat cell lysate. Human intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab23341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceNuclear receptor coactivator NCoA62 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Bx 42 antibody
    • Bx42 antibody
    • Homolog of Drosophila BX42 antibody
    • MGC119379 antibody
    • NCOA 62 antibody
    • Nuclear protein SkiP antibody
    • Nuclear receptor coactivator 62 kD antibody
    • Nuclear receptor coactivator NCoA 62 antibody
    • Nuclear receptor coactivator NCoA62 antibody
    • Prp 45 antibody
    • Prp45 antibody
    • PRPF 45 antibody
    • PRPF45 antibody
    • Ski interacting protein antibody
    • Ski-interacting protein antibody
    • SKIIP antibody
    • SKIP antibody
    • SNW 1 antibody
    • SNW domain containing 1 antibody
    • SNW domain containing protein 1 antibody
    • SNW1 antibody
    • SNW1 protein antibody
    see all

Anti-NCOA62 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling NCOA62 with ab23341 at a concentration of 4-8µg/ml. Positive cells marked by arrows are epithelial cells of intestinal villus. Magnification: X400.

  • Anti-NCOA62 antibody (ab23341) at 1 µg/ml + Jurkat cell lysate.

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000.

    Predicted band size : 59 kDa
    Observed band size : 61 kDa (why is the actual band size different from the predicted?)

References for Anti-NCOA62 antibody (ab23341)

ab23341 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293T)
Total protein in input 500 µg
Specification 293T
Immuno-precipitation step Protein A
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Submitted Oct 30 2007

Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 40 µg
Specification 293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 25 2007

Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 40 µg
Specification PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

Submitted Jul 13 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"