The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceNuclear receptor coactivator NCoA62 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling NCOA62 with ab23341 at a concentration of 4-8µg/ml. Positive cells marked by arrows are epithelial cells of intestinal villus. Magnification: X400.
Western blot - NCOA62 antibody (ab23341)
Anti-NCOA62 antibody (ab23341) at 1 µg/ml + Jurkat cell lysate.
Secondary HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000.