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Anti-NCoR2 antibody [1212] (ab2780)

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Overview

Product name

Anti-NCoR2 antibody [1212]
See all NCOR2 products (7) ...

Description

Mouse monoclonal [1212] to NCoR2

Specificity

We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Tested applications

IHC-Fr, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: CGGA-SASDPHREKTQ, corresponding to amino acids 994-1005 of Human NCoR 2.

CGGA-SASDP HREKTQ

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, 1 mg/ml BSA, 0.05% sodium azide

Concentration

Concentration information loading...

Purity

IgG fraction

Primary antibody notes

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing Mediator of Retinoic acid and Thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

Clonality

Monoclonal

Clone number

1212

Isotype

IgG1

  • Immunocytochemistry/ Immunofluorescence - NCoR2 antibody [1212] (ab2780)Immunocytochemistry/ Immunofluorescence - NCoR2 antibody [1212] (ab2780) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-Fr

     IHC-Fr: 1/50

    IHC-Fr: 1/50

  • ShowHide

    WB

     WB: 1/250Detects a band ...Read more →

    WB: 1/250Detects a band of approximately 220-250 kDa (predicted molecular weight: 274 kDa).

Target

Function

Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.

Tissue specificity

Ubiquitous. High levels of expression are detected in lung, spleen and brain.

Sequence similarities

Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains.

Domain

The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9Y618 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CTG repeat protein 26 antibody
  • retinoic-acid-receptor-associated corepressor antibody
  • SMAP270 antibody
  • T3 receptor associating factor antibody
  • Thyroid retinoic acid receptor associated corepressor antibody
  • TRAC antibody
  • CTG 26 antibody
  • CTG repeat protein 26 antibody
  • CTG26 antibody
  • N CoR2 antibody
  • N-CoR2 antibody
  • NCoR 2 antibody
  • NCoR 2 antibody
  • Ncor2 antibody
  • NCOR2_HUMAN antibody
  • nuclear receptor co-repressor 2 antibody
  • Nuclear receptor corepressor 2 antibody
  • Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody
  • Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody
  • Silencing mediator of retinoic acid and thyroid hormone receptor antibody
  • SMAP 270 antibody
  • SMAP270 antibody
  • SMRT antibody
  • SMRTE antibody
  • SMRTE tau antibody
  • T3 receptor-associating factor antibody
  • Thyroid retinoic acid receptor associated corepressor antibody
  • Thyroid- antibody
  • Thyroid- retinoic-acid-receptor-associated corepressor antibody
  • TNRC 14 antibody
  • TNRC14 antibody
  • TRAC 1 antibody
  • TRAC antibody
  • TRAC1 antibody
see all

Anti-NCoR2 antibody [1212] images:

  Immunocytochemistry/ Immunofluorescence - NCoR2 antibody [1212] (ab2780)

Immunocytochemistry/ Immunofluorescence - NCoR2 antibody [1212] (ab2780)

Immunolocalization of SMRT in HeLa cells using ab2780. 

References for Anti-NCoR2 antibody [1212] (ab2780)

This product has been referenced in:

  • Yu Jet al. The corepressor silencing mediator for retinoid and thyroid hormone receptor facilitates cellular recovery from DNA double-strand breaks. Cancer Res 66:9316-22 (2006). WB.Read more (PubMed: 16982777) »
  • Mani Set al. Activation of the steroid and xenobiotic receptor (human pregnane X receptor) by nontaxane microtubule-stabilizing agents. Clin Cancer Res 11:6359-69 (2005).Read more (PubMed: 16144941) »

See all 2 publications for this product

Publishing research using ab2780? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"