Anti-NCR1 antibody [MM0491-8F24] (ab89877)

Overview

  • Product name
    Anti-NCR1 antibody [MM0491-8F24]
    See all NCR1 primary antibodies
  • Description
    Mouse monoclonal [MM0491-8F24] to NCR1
  • Tested applications
    Suitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the extracellular domain of Human NCR1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and sterile filtered through a 0.2 µm filter.
  • Clonality
    Monoclonal
  • Clone number
    MM0491-8F24
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IHC-Fr 1/20 - 1/100.
Flow Cyt 1/50 - 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Tissue specificity
    Selectively expressed by both resting and activated NK cells.
  • Sequence similarities
    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    N-glycosylated.
    O-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating NK receptor NK p46 antibody
    • CD335 antibody
    • FLJ99094 antibody
    • hNKp46 antibody
    • Ly94 antibody
    • Lymphocyte antigen 94 antibody
    • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
    • Lymphocyte antigen 94 homolog antibody
    • Natural cytotoxicity triggering receptor 1 antibody
    • Natural killer cell p46-related protein antibody
    • NCR1 antibody
    • NCT1 antibody
    • NCTR1_HUMAN antibody
    • NK cell activating receptor antibody
    • NK cell-activating receptor antibody
    • NK-p46 antibody
    • NKp46 antibody
    see all

References for Anti-NCR1 antibody [MM0491-8F24] (ab89877)

ab89877 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization
No
Gating Strategy
lymphocytes based on SSC vs FSC
Specification
peripheral blood mononuclear cells (PBMC)
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Osteosarcoma)
Permeabilization
No
Specification
Osteosarcoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 25 2015

Application
Flow Cytometry
Sample
Human Cell (PBMCs)
Permeabilization
No
Gating Strategy
Live cell and PBMC population
Specification
PBMCs
Fixation
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 16 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (NK cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NK cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Jun 02 2015

Thank you for contacting Abcam.

For ab89877, the isotype is IgG2b.

If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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