Anti-NCR1 antibody [MM0491-8F24] (ab89877)

Overview

  • Product nameAnti-NCR1 antibody [MM0491-8F24]
    See all NCR1 primary antibodies
  • Description
    Mouse monoclonal [MM0491-8F24] to NCR1
  • Tested applicationsSuitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the extracellular domain of Human NCR1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThe IgG fraction of culture supernatant was purified by Protein G affinity chromatography and sterile filtered through a 0.2 µm filter.
  • ClonalityMonoclonal
  • Clone numberMM0491-8F24
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IHC-Fr 1/20 - 1/100.
Flow Cyt 1/50 - 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Tissue specificitySelectively expressed by both resting and activated NK cells.
  • Sequence similaritiesBelongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    N-glycosylated.
    O-glycosylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating NK receptor NK p46 antibody
    • CD335 antibody
    • FLJ99094 antibody
    • hNKp46 antibody
    • Ly94 antibody
    • Lymphocyte antigen 94 antibody
    • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
    • Lymphocyte antigen 94 homolog antibody
    • Natural cytotoxicity triggering receptor 1 antibody
    • Natural killer cell p46-related protein antibody
    • NCR1 antibody
    • NCT1 antibody
    • NCTR1_HUMAN antibody
    • NK cell activating receptor antibody
    • NK cell-activating receptor antibody
    • NK-p46 antibody
    • NKp46 antibody
    see all

References for Anti-NCR1 antibody [MM0491-8F24] (ab89877)

ab89877 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization No
Gating Strategy lymphocytes based on SSC vs FSC
Specification peripheral blood mononuclear cells (PBMC)
Fixation Paraformaldehyde
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Submitted Oct 14 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Osteosarcoma)
Permeabilization No
Specification Osteosarcoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative Formaldehyde
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Submitted Jun 25 2015

Application Flow Cytometry
Sample Human Cell (PBMCs)
Permeabilization No
Gating Strategy Live cell and PBMC population
Specification PBMCs
Fixation Formaldehyde
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Submitted Jun 16 2015

Application Western blot
Sample Human Cell lysate - whole cell (NK cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification NK cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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Submitted Jun 02 2015

Thank you for contacting Abcam.

For ab89877, the isotype is IgG2b.

If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"