Anti-NCR3 antibody [y4E6] (ab26250)

Overview

  • Product name
    Anti-NCR3 antibody [y4E6]
    See all NCR3 primary antibodies
  • Description
    Mouse monoclonal [y4E6] to NCR3
  • Specificity
    This antibody is specific for NCR3 and does not cross react with NKp46 and NKp44
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: extracellular domain(19-138aa) of human NCR3 purified from E. coli.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    y4E6
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/2000. Predicted molecular weight: 22 kDa.

Target

  • Function
    Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation.
  • Tissue specificity
    Selectively expressed by all resting and activated NK cells and weakly expressed in spleen.
  • Sequence similarities
    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1C7 antibody
    • Activating natural killer receptor p30 antibody
    • Activating NK A1 receptor antibody
    • CD337 antibody
    • CD337 antigen antibody
    • LY117 antibody
    • Lymphocyte antigen 117 antibody
    • MALS antibody
    • Natural cytotoxicity triggering receptor 3 antibody
    • Natural killer cell p30 related protein antibody
    • Natural killer cell p30-related protein antibody
    • Ncr3 antibody
    • NCTR3_HUMAN antibody
    • NK-p30 antibody
    • NKp30 antibody
    see all

Images

  • Anti-NCR3 antibody [y4E6] (ab26250) at 1/1000 dilution + human recombinant protein NCR3 (residues 19-138) (50ng)

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size : 22 kDa

References

ab26250 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
spleen
Blocking step
ab80436 protein block as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 22 2016

Thank you for your enquiry. To answer your questions: 1. Unfortunately we do not sell the NKp30 receptor in solution. 2) A recombinant fragment of the extracellular domain(19-138aa) of human NCR3 was used as the immunogen for anti-NKp30 (ab26...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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