Overview

  • Product nameAnti-NCS1 antibody
    See all NCS1 primary antibodies
  • Description
    Rabbit polyclonal to NCS1
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein Mouse NCS1 protein with extensive post-translational modifications.

  • Positive control
    • Human brain, cerebellum tissue
  • General notesDilute only prior to immediate use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionNeuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
  • Sequence similaritiesBelongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Cellular localizationGolgi apparatus > Golgi stack membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cytoplasm > perinuclear region. Cell membrane. Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9430075O15Rik antibody
    • A730032G13Rik antibody
    • AI836659 antibody
    • DKFZp761L1223 antibody
    • FLUP antibody
    • FREQ antibody
    • Frequenin antibody
    • Frequenin homolog (Drosophila) antibody
    • Frequenin homolog antibody
    • Frequenin like protein antibody
    • Frequenin, Drosophila, homolog of antibody
    • Frequenin-like protein antibody
    • Frequenin-like ubiquitous protein antibody
    • Mfreq antibody
    • NCS 1 antibody
    • NCS-1 antibody
    • ncs1 antibody
    • NCS1_HUMAN antibody
    • Neuronal calcium sensor 1 antibody
    • Neuronal Calicum Sensor 1 antibody
    see all

Anti-NCS1 antibody images

  • ab116230, at 5 µg/ml, staining NCS1 in formalin fixed, paraffin embedded Human brain (cerebellum) tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-NCS1 antibody (ab116230)

ab116230 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"