Overview

  • Product nameAnti-NDC1 antibody
  • Description
    Rabbit polyclonal to NDC1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 323-372 (PPIIKYLALQ DLMLLSQYSP SRRQEVFSLS QPGGHPHNWT AISRECLNLL) of Human NDC1, NP_060557.

  • Positive control
    • Fetal lung lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75, >90 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceNDC1 is a component of the nuclear pore complex (NPC), which plays a key role in de novo assembly and insertion of NPC in the nuclear envelope. NDC1 is required for NPC and nuclear envelope assembly, possibly by forming a link between the nuclear envelope membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane. NDC1 colocalizes with p62 (NUP62) on the nuclear envelope at the nuclear rim. The protein has 6 transmembrane domains and several putative phosphorylation sites.
  • Cellular localizationNucleus; nuclear pore complex; multipass membrane protein
  • Database links
  • Alternative names
    • FLJ10407 antibody
    • FLJ12556 antibody
    • FLJ34120 antibody
    • hNDC1 antibody
    • TMEM48 antibody
    • Transmembrane protein 48 antibody
    see all

Anti-NDC1 antibody images

  • Anti-NDC1 antibody (ab83876) at 1 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 75,>90 kDa (why is the actual band size different from the predicted?)

References for Anti-NDC1 antibody (ab83876)

This product has been referenced in:
  • Tarazón E  et al. Heart failure induces significant changes in nuclear pore complex of human cardiomyocytes. PLoS One 7:e48957 (2012). WB ; Human . Read more (PubMed: 23152829) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"