Overview

  • Product nameAnti-NDE1 antibody
    See all NDE1 primary antibodies
  • Description
    Rabbit polyclonal to NDE1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region of Human NDE1

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRequired for centrosome duplication. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex (By similarity). Required for formation and function of the mitotic spindle.
  • Sequence similaritiesBelongs to the nudE family.
  • Post-translational
    modifications
    Phosphorylated in mitosis.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Localizes to the interphase centrosome and to the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20101 antibody
    • HOM TES 87 antibody
    • LIS1 interacting protein NUDE1 rat homolog antibody
    • LIS1 interacting protein NUDE1 antibody
    • LIS4 antibody
    • NDE 1 antibody
    • NDE1 antibody
    • NDE1_HUMAN antibody
    • Nuclear distribution gene E homolog 1 antibody
    • Nuclear distribution protein nudE homolog 1 antibody
    • NUDE 1 antibody
    • NudE antibody
    • NudE nuclear distribution gene E homolog 1 (A. nidulans) antibody
    • NudE nuclear distribution gene E homolog 1 antibody
    • NUDE1 antibody
    see all

Anti-NDE1 antibody images

  • Anti-NDE1 antibody (ab76384) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References for Anti-NDE1 antibody (ab76384)

ab76384 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"