Overview

  • Product name
  • Description
    Mouse polyclonal to NDFIP2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NDFIP2 full-length human protein

  • Positive control
    • NDFIP2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Target

  • Function
    Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling.
  • Tissue specificity
    Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta.
  • Domain
    The PY (WW-binding) motifs are required for E3 ubiquitin-protein ligase activation and for ubiquitination.
  • Post-translational
    modifications
    Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover.
    Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Also phosphorylated by LYN and FYN.
  • Cellular localization
    Endosome membrane. Golgi apparatus membrane. Endosome > multivesicular body membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ndfip2 antibody
    • NEDD4 family interacting protein 2 antibody
    • NEDD4 family-interacting protein 2 antibody
    • NEDD4 WW domain binding protein 5A antibody
    • NEDD4 WW domain-binding protein 5A antibody
    • NFIP2_HUMAN antibody
    • Putative MAPK activating protein PM04/PM05/PM06/PM07 antibody
    • Putative MAPK-activating protein PM04/PM05/PM06/PM07 antibody
    • Putative NF kappa B activating protein 413 antibody
    • Putative NF-kappa-B-activating protein 413 antibody
    see all

Images

  • All lanes : Anti-NDFIP2 antibody (ab67912) at 1/500 dilution

    Lane 1 : NDFIP2 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

References

ab67912 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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