Overview

  • Product name
    Anti-NDNF antibody - N-terminal
    See all NDNF primary antibodies
  • Description
    Rabbit polyclonal to NDNF - N-terminal
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NDNF aa 87-117 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_078850.3.
    Database link: Q8TB73

  • Positive control
    • Human lung carcinoma tissue. 293 cells and cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175602 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 65 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Promotes matrix assembly and cell adhesiveness (By similarity). Promotes neuron migration, growth and survival as well as neurite outgrowth.
  • Sequence similarities
    Contains 2 fibronectin type-III domains.
  • Post-translational
    modifications
    O-glycosylated; contains heparan sulfate and chondroitin sulfate.
    N-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C4orf31 antibody
    • Fibronectin type III domain containing protein C4orf31 antibody
    • NDNF antibody
    • NDNF_HUMAN antibody
    • Neuron-derived neurotrophic factor antibody
    • PRO6487 antibody
    • Protein NDNF antibody
    • UNQ2748 antibody
    see all

Images

  • Anti-NDNF antibody - N-terminal (ab175602) at 1/1000 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 65 kDa
  • Immunohistochemistry analysis of formalin-fixed paraffin-embedded Human lung carcinoma tissue labeling NDNF with ab175602 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometry analysis of 293 cells labeling NDNF with ab175602 at 1/10 (right histogram) and a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175602 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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