Anti-NDRG1 antibody (ab196621)
Key features and details
- Rabbit polyclonal to NDRG1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NDRG1 antibody
See all NDRG1 primary antibodies -
Description
Rabbit polyclonal to NDRG1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human NDRG1 (C terminal).
Database link: Q92597 -
Positive control
- U251, HeLa, SW480, PC3, mouse kidney,eye and kidney cell extracts. Mouse lung, rat brain and rat kidney tissue. MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 43 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 43 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
May have a growth inhibitory role. -
Tissue specificity
Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression. -
Involvement in disease
Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. -
Sequence similarities
Belongs to the NDRG family. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro. - Information by UniProt
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Database links
- Entrez Gene: 10397 Human
- Entrez Gene: 17988 Mouse
- Entrez Gene: 299923 Rat
- Omim: 605262 Human
- SwissProt: Q92597 Human
- SwissProt: Q62433 Mouse
- SwissProt: Q6JE36 Rat
- Unigene: 372914 Human
see all -
Alternative names
- 42 kDa antibody
- Anti GC4 antibody
- cap43 antibody
see all
Images
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All lanes : Anti-NDRG1 antibody (ab196621) at 1/50 dilution
Lane 1 : U251 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SW480 cell extract
Lane 4 : PC3 cell extract
Lane 5 : Mouse kidney cell extract
Lane 6 : Mouse eye cell extract
Lane 7 : Mouse brain cell extract
Developed using the ECL technique.
Predicted band size: 43 kDa -
Immunofluorescence analysis of MCF7 cells labeling NDRG1 with ab196621 at a 1/50 dilution. Counter-stained with DAPI (blue) for nuclear staining.
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Immunohistochemical analysis of formalin fixed and paraffin embedded rat kidney tissue labeling NDRG1 with ab196621 at a 1/100 dilution.
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Immunohistochemical analysis of formalin fixed and paraffin embedded rat brain tissue labeling NDRG1 with ab196621 at a 1/100 dilution.
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Immunohistochemical analysis of formalin fixed and paraffin embedded rat lung tissue labeling NDRG1 with ab196621 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab196621 has been referenced in 2 publications.
- Wang J et al. Hsa_circ_0003159 inhibits gastric cancer progression by regulating miR-223-3p/NDRG1 axis. Cancer Cell Int 20:57 (2020). PubMed: 32099530
- Lee E et al. Reduction of two histone marks, H3k9me3 and H3k27me3 by epidrug induces neuroendocrine differentiation in prostate cancer. J Cell Biochem 119:3697-3705 (2018). PubMed: 29236331