The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 43 kDa.
1/3200. Peptide ELISA
Use at an assay dependent concentration.
May have a growth inhibitory role.
Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
Involvement in disease
Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
Belongs to the NDRG family.
Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
N myc downstream regulated gene 1 protein antibody
N-myc downstream-regulated gene 1 protein antibody
Ndr 1 antibody
NDRG 1 antibody
NDRG1 protein antibody
Nickel specific induction protein antibody
Nickel specific induction protein Cap43 antibody
Nickel-specific induction protein Cap43 antibody
Nmyc downstream regulated antibody
Nmyc downstream regulated gene1 antibody
Nmyc downstream regulated gene1 protein antibody
Protein NDRG1 antibody
Protein regulated by oxygen 1 antibody
Protein regulated by oxygen1 antibody
Reduced in tumor antibody
Reducing agents and tunicamycin responsive protein antibody
Reducing agents and tunicamycin-responsive protein antibody
Tunicamycin responsive protein antibody
Western blot - Anti-NDRG1 antibody (ab37897)
Anti-NDRG1 antibody (ab37897) at 0.1 µg/ml + Human Kidney lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size : 43 kDa Observed band size : 48 kDa (why is the actual band size different from the predicted?) Primary incubated for 1 hour. Detected by western blot using chemiluminescence. A minor band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide.
Drelon C et al. PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development. Nat Commun7:12751 (2016).
Read more (PubMed: 27624192) »
Kovacevic Z et al. The Metastasis Suppressor, N-MYC Downstream-regulated Gene-1 (NDRG1), Down-regulates the ErbB Family of Receptors to Inhibit Downstream Oncogenic Signaling Pathways. J Biol Chem291:1029-52 (2016).
Read more (PubMed: 26534963) »