Overview

  • Product name
  • Description
    Goat polyclonal to NDRG1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    GNSAGPKSMEVSC

    , corresponding to C terminal amino acids 382-394 of Human NDRG1

Properties

Applications

Our Abpromise guarantee covers the use of ab37897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 43 kDa.
ELISA 1/3200. Peptide ELISA
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May have a growth inhibitory role.
  • Tissue specificity
    Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
  • Involvement in disease
    Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
  • Sequence similarities
    Belongs to the NDRG family.
  • Cellular localization
    Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
  • Information by UniProt
  • Database links
  • Alternative names
    • 42 kDa antibody
    • Anti GC4 antibody
    • cap43 antibody
    • cmt4d antibody
    • Differentiation related gene1 protein antibody
    • Differentiation-related gene 1 protein antibody
    • Drg 1 antibody
    • DRG-1 antibody
    • drg1 antibody
    • gc4 antibody
    • GC4 antibody antibody
    • hmsnl antibody
    • Human mRNA for RTP complete cds antibody
    • N myc downstream regulated gene 1 antibody
    • N myc downstream regulated gene 1 protein antibody
    • N-myc downstream-regulated gene 1 protein antibody
    • Ndr 1 antibody
    • ndr1 antibody
    • NDRG 1 antibody
    • Ndrg1 antibody
    • NDRG1 protein antibody
    • NDRG1_HUMAN antibody
    • Nickel specific induction protein antibody
    • Nickel specific induction protein Cap43 antibody
    • Nickel-specific induction protein Cap43 antibody
    • nmsl antibody
    • Nmyc downstream regulated antibody
    • Nmyc downstream regulated gene1 antibody
    • Nmyc downstream regulated gene1 protein antibody
    • Protein NDRG1 antibody
    • Protein regulated by oxygen 1 antibody
    • Protein regulated by oxygen1 antibody
    • Proxy1 antibody
    • Reduced in tumor antibody
    • Reducin antibody
    • Reducing agents and tunicamycin responsive protein antibody
    • Reducing agents and tunicamycin-responsive protein antibody
    • Rit42 antibody
    • RTP antibody
    • targ1 antibody
    • TDD5 antibody
    • tdds antibody
    • Tunicamycin responsive protein antibody
    see all

Anti-NDRG1 antibody images

  • Anti-NDRG1 antibody (ab37897) at 0.1 µg/ml + Human Kidney lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 43 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Primary incubated for 1 hour. Detected by western blot using chemiluminescence. A minor band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide.

References for Anti-NDRG1 antibody (ab37897)

This product has been referenced in:
  • Drelon C  et al. PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development. Nat Commun 7:12751 (2016). IHC-P ; Mouse . Read more (PubMed: 27624192) »
  • Kovacevic Z  et al. The Metastasis Suppressor, N-MYC Downstream-regulated Gene-1 (NDRG1), Down-regulates the ErbB Family of Receptors to Inhibit Downstream Oncogenic Signaling Pathways. J Biol Chem 291:1029-52 (2016). WB ; Human . Read more (PubMed: 26534963) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (liver)
Loading amount
30 µg
Specification
liver
Treatment
hypoxia treatment for 24h
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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