Overview

  • Product nameAnti-NDRG1 antibody [EPR5593]
    See all NDRG1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5593] to NDRG1
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids of Human NDRG1.

  • Positive control
    • Caco 2, Human fetal kidney, LnCaP, and HeLa cell lysates; Human colon tissue; HeLa cells.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionMay have a growth inhibitory role.
    • Tissue specificityUbiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
    • Involvement in diseaseDefects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
    • Sequence similaritiesBelongs to the NDRG family.
    • Cellular localizationCytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
    • Information by UniProt
    • Database links
    • Alternative names
      • 42 kDa antibody
      • Anti GC4 antibody
      • cap43 antibody
      • cmt4d antibody
      • Differentiation related gene1 protein antibody
      • Differentiation-related gene 1 protein antibody
      • Drg 1 antibody
      • DRG-1 antibody
      • drg1 antibody
      • gc4 antibody
      • GC4 antibody antibody
      • hmsnl antibody
      • Human mRNA for RTP complete cds antibody
      • N myc downstream regulated gene 1 antibody
      • N myc downstream regulated gene 1 protein antibody
      • N-myc downstream-regulated gene 1 protein antibody
      • Ndr 1 antibody
      • ndr1 antibody
      • NDRG 1 antibody
      • Ndrg1 antibody
      • NDRG1 protein antibody
      • NDRG1_HUMAN antibody
      • Nickel specific induction protein antibody
      • Nickel specific induction protein Cap43 antibody
      • Nickel-specific induction protein Cap43 antibody
      • nmsl antibody
      • Nmyc downstream regulated antibody
      • Nmyc downstream regulated gene1 antibody
      • Nmyc downstream regulated gene1 protein antibody
      • Protein NDRG1 antibody
      • Protein regulated by oxygen 1 antibody
      • Protein regulated by oxygen1 antibody
      • Proxy1 antibody
      • Reduced in tumor antibody
      • Reducin antibody
      • Reducing agents and tunicamycin responsive protein antibody
      • Reducing agents and tunicamycin-responsive protein antibody
      • Rit42 antibody
      • RTP antibody
      • targ1 antibody
      • TDD5 antibody
      • tdds antibody
      • Tunicamycin responsive protein antibody
      see all

    Anti-NDRG1 antibody [EPR5593] images

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • ab124689 at 1/100 dilution, staining NDRG1 in Formalin fixed Paraffin-embedded Human colon tissue by Immunohistochemistry.
    • All lanes : Anti-NDRG1 antibody [EPR5593] (ab124689) at 1/10000 dilution

      Lane 1 : CaCo 2 lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : LnCaP lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 43 kDa
    • ab124689, at 1/100 dilution, staining NDRG1 in HeLa cells by Immunofluorescence.
    • ab124689 showing positive staining in Cervical carcinoma tissue.

    • ab124689 showing positive staining in Colonic adenocarcinoma tissue.

    • ab124689 showing positive staining in Normal kidney tissue.

    • ab124689 showing positive staining in Breast carcinoma tissue.

    References for Anti-NDRG1 antibody [EPR5593] (ab124689)

    This product has been referenced in:
    • Lee JE & Kim JH Valproic acid inhibits the invasion of PC3 prostate cancer cells by upregulating the metastasis suppressor protein NDRG1. Genet Mol Biol 38:527-33 (2015). WB ; Human . Read more (PubMed: 26692161) »
    • Lee JE & Kim JH SUMO modification regulates the protein stability of NDRG1. Biochem Biophys Res Commun 459:161-5 (2015). WB ; Human . Read more (PubMed: 25712528) »

    See all 6 Publications for this product

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