Recombinant
RabMAb

Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)

Overview

  • Product name
    Anti-NDRG1 (phospho S330) antibody [EPR5594]
    See all NDRG1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5594] to NDRG1 (phospho S330)
  • Specificity
    This antibody only detects NDRG1 when phosphorylated at Serine 330.
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 330 of Human NDRG1

  • Positive control
    • Calyculin A treated 293T cell lysates; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      May have a growth inhibitory role.
    • Tissue specificity
      Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
    • Involvement in disease
      Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
    • Sequence similarities
      Belongs to the NDRG family.
    • Cellular localization
      Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
    • Information by UniProt
    • Database links
    • Alternative names
      • 42 kDa antibody
      • Anti GC4 antibody
      • cap43 antibody
      • cmt4d antibody
      • Differentiation related gene1 protein antibody
      • Differentiation-related gene 1 protein antibody
      • Drg 1 antibody
      • DRG-1 antibody
      • drg1 antibody
      • gc4 antibody
      • GC4 antibody antibody
      • hmsnl antibody
      • Human mRNA for RTP complete cds antibody
      • N myc downstream regulated gene 1 antibody
      • N myc downstream regulated gene 1 protein antibody
      • N-myc downstream-regulated gene 1 protein antibody
      • Ndr 1 antibody
      • ndr1 antibody
      • NDRG 1 antibody
      • Ndrg1 antibody
      • NDRG1 protein antibody
      • NDRG1_HUMAN antibody
      • Nickel specific induction protein antibody
      • Nickel specific induction protein Cap43 antibody
      • Nickel-specific induction protein Cap43 antibody
      • nmsl antibody
      • Nmyc downstream regulated antibody
      • Nmyc downstream regulated gene1 antibody
      • Nmyc downstream regulated gene1 protein antibody
      • Protein NDRG1 antibody
      • Protein regulated by oxygen 1 antibody
      • Protein regulated by oxygen1 antibody
      • Proxy1 antibody
      • Reduced in tumor antibody
      • Reducin antibody
      • Reducing agents and tunicamycin responsive protein antibody
      • Reducing agents and tunicamycin-responsive protein antibody
      • Rit42 antibody
      • RTP antibody
      • targ1 antibody
      • TDD5 antibody
      • tdds antibody
      • Tunicamycin responsive protein antibody
      see all

    Images

    • All lanes : Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713) at 1/10000 dilution

      Lane 1 : Untreated 293T cell lysates
      Lane 2 : Calyculin A treated 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 43 kDa
    • ab124713, at 1/100, staining NDRG1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Costin BN  et al. Ethanol regulation of serum glucocorticoid kinase 1 expression in DBA2/J mouse prefrontal cortex. PLoS One 8:e72979 (2013). Read more (PubMed: 23991167) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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