Overview

  • Product nameAnti-NDRG2 antibody
    See all NDRG2 primary antibodies
  • Description
    Rabbit polyclonal to NDRG2
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 300-350 (GYMASSCMTR LSRSRTASLT SAASVDGNRS RSRTLSQSSE SGTLSSGPPG) of Human NDRG2 (NP_057334; UniProt Q9UN36 isoform 2).

  • Positive control
    • WB: HepG2 cell lysate IHC-P: Human liver lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: U87-MG.

Properties

Applications

Our Abpromise guarantee covers the use of ab118927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionMay be involved in dendritic cell and neuron differentiation. May have anti-tumor activity.
  • Tissue specificityHighly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).
  • Sequence similaritiesBelongs to the NDRG family.
  • Developmental stageSpecifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development.
  • Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antidepressant related protein ADRG123 antibody
    • Cytoplasmic protein Ndr1 antibody
    • DKFZp781G1938 antibody
    • FLJ25522 antibody
    • KIAA1248 antibody
    • N myc downstream regulated gene 2 antibody
    • N myc downstream regulator 2 antibody
    • NDR1 related protein NDR2 antibody
    • NDRG 2 antibody
    • NDRG family member 2 antibody
    • NDRG1 related protein antibody
    • NDRG2 antibody
    • NDRG2_HUMAN antibody
    • Protein NDRG2 antibody
    • Protein Syld709613 antibody
    • SYLD antibody
    • Syld709613 protein antibody
    see all

Anti-NDRG2 antibody images

  • ICC/IF image of ab118927 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab118927 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-NDRG2 antibody (ab118927) at 1 µg/ml

    Lane 1 : Mwt marker
    Lane 2 : HepG2 cell lysate at 10 µg


    Predicted band size : 39 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling NDRG2 with ab118927 at 5µg/ml.

References for Anti-NDRG2 antibody (ab118927)

ab118927 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"