Anti-NDRG3 antibody - C-terminal (ab176695)
Key features and details
- Rabbit polyclonal to NDRG3 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NDRG3 antibody - C-terminal
See all NDRG3 primary antibodies -
Description
Rabbit polyclonal to NDRG3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human NDRG3 aa 350-375 (N terminal). The exact sequence is proprietary.
Database link: Q9UGV2 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 41 kDa. |
Target
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Tissue specificity
Ubiquitous. Highly expressed in brain. -
Sequence similarities
Belongs to the NDRG family. - Information by UniProt
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Database links
- Entrez Gene: 735544 Chimpanzee
- Entrez Gene: 514399 Cow
- Entrez Gene: 57446 Human
- Entrez Gene: 29812 Mouse
- Entrez Gene: 100172705 Orangutan
- Entrez Gene: 296318 Rat
- Omim: 605273 Human
- SwissProt: A7MB28 Cow
see all -
Alternative names
- FLJ13556 antibody
- N myc downstream regulated gene 3 antibody
- N-myc downstream-regulated gene 3 protein antibody
see all
Images
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All lanes : Anti-NDRG3 antibody - C-terminal (ab176695) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : 293T whole cell lysates at 50 µg
Lane 4 : Jurkat whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds -
Immunoprecipitation. Lane 1: labeling of NDRG3 in HeLa whole cell lysate immunoprecipitated using ab176695 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab176695 has not yet been referenced specifically in any publications.