• Product name
  • Description
    Mouse polyclonal to NDUFA5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Fusion protein:


    , corresponding to amino acids 43/116 of Mouse NDUFA5

  • General notes
    Produced from outbred CD1 mice

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.



Our Abpromise guarantee covers the use of ab22096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 13 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.


  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Tissue specificity
    Expressed in all tissues examined with highest levels in heart, skeletal muscle and brain.
  • Sequence similarities
    Belongs to the complex I NDUFA5 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B13 antibody
    • CI 13kB antibody
    • CI-13kD-B antibody
    • Complex I subunit B13 antibody
    • Complex I-13kD-B antibody
    • DKFZp781K1356 antibody
    • FLJ12147 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (13kD, B13) antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 antibody
    • NADH-ubiquinone oxidoreductase 13 kDa-B subunit antibody
    • NDUA5_HUMAN antibody
    • NDUFA5 antibody
    • NUFM antibody
    • Type I dehydrogenase antibody
    • Ubiquinone reductase antibody
    • UQOR13 antibody
    see all

References for Anti-NDUFA5 antibody (ab22096)

ab22096 has not yet been referenced specifically in any publications.

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