Overview

  • Product nameAnti-NDUFA8 antibody
    See all NDUFA8 primary antibodies
  • Description
    Mouse monoclonal to NDUFA8
  • Tested applicationsSuitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1 -73 of Human NDUFA8 with a proprietary (NP_055037).

  • Positive control
    • NDUFA8 transfected 293T cell line lysate; Human heart tissue.

Applications

Our Abpromise guarantee covers the use of ab118319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 3 µg/ml.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similaritiesBelongs to the complex I NDUFA8 subunit family.
    Contains 2 CHCH domains.
  • DomainContains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
  • Post-translational
    modifications
    May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.
  • Cellular localizationMitochondrion. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI 19KD antibody
    • CI PGIV antibody
    • CI-19kD antibody
    • CI-PGIV antibody
    • Complex I 19k antibody
    • Complex I 19kD antibody
    • Complex I PGIV antibody
    • Complex I PGIV subunit antibody
    • Complex I-19kD antibody
    • Complex I-PGIV antibody
    • MGC793 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 (19kD PGIV) antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 19kDa antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 antibody
    • NADH ubiquinone oxidoreductase 19 kDa subunit antibody
    • NADH ubiquinone oxidoreductase PGIV subunit antibody
    • NADH-ubiquinone oxidoreductase 19 kDa subunit antibody
    • NDUA8_HUMAN antibody
    • NDUFA 8 antibody
    • NDUFA8 antibody
    • OTTHUMP00000022034 antibody
    • PGIV antibody
    see all

Anti-NDUFA8 antibody images

  • Anti-NDUFA8 antibody (ab118319) at 5 µg/ml + recombinant immunogen at 0.2 µg

    Predicted band size : 20 kDa
  • All lanes : Anti-NDUFA8 antibody (ab118319) at 5 µg/ml

    Lane 1 : NDUFA8 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 20 kDa
  • ab118319, at 3 ug/ml, staining NDUFA8 in formalin-fixed, paraffin-embedded Human heart tissue by Immunohistochemistry.
  • Detection limit for recombinant tagged NDUFA8 is 0.03 ng/ml as a capture antibody.

References for Anti-NDUFA8 antibody (ab118319)

ab118319 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"