Overview

  • Product nameAnti-NDUFA8 antibody
    See all NDUFA8 primary antibodies
  • Description
    Rabbit polyclonal to NDUFA8
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human NDUFA8.

  • Positive control
    • Human pancreas tissue. Extracts from MCF-7 cells, 293 cells, HUVEC cells, HepG2 cells and Jurkat cells.

Associated products

Applications

Our Abpromise guarantee covers the use of ab74126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

  • FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similaritiesBelongs to the complex I NDUFA8 subunit family.
    Contains 2 CHCH domains.
  • DomainContains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
  • Post-translational
    modifications
    May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.
  • Cellular localizationMitochondrion. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI 19KD antibody
    • CI PGIV antibody
    • CI-19kD antibody
    • CI-PGIV antibody
    • Complex I 19k antibody
    • Complex I 19kD antibody
    • Complex I PGIV antibody
    • Complex I PGIV subunit antibody
    • Complex I-19kD antibody
    • Complex I-PGIV antibody
    • MGC793 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 (19kD PGIV) antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 19kDa antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 antibody
    • NADH ubiquinone oxidoreductase 19 kDa subunit antibody
    • NADH ubiquinone oxidoreductase PGIV subunit antibody
    • NADH-ubiquinone oxidoreductase 19 kDa subunit antibody
    • NDUA8_HUMAN antibody
    • NDUFA 8 antibody
    • NDUFA8 antibody
    • OTTHUMP00000022034 antibody
    • PGIV antibody
    see all

Anti-NDUFA8 antibody images

  • Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using ab74126, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • All lanes : Anti-NDUFA8 antibody (ab74126) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from HUVEC cells
    Lane 4 : Extracts from HepG2 cells
    Lane 5 : Extracts from Jurkat cells
    Lane 6 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

References for Anti-NDUFA8 antibody (ab74126)

ab74126 has not yet been referenced specifically in any publications.

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