The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
1/50 - 1/100.
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I NDUFA8 subunit family. Contains 2 CHCH domains.
DomainContains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
Post-translational modificationsMay contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using ab74126, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Western blot - NDUFA8 antibody (ab74126)
All lanes : Anti-NDUFA8 antibody (ab74126) at 1/500 dilution
Lane 1 : Extracts from MCF-7 cells Lane 2 : Extracts from 293 cells Lane 3 : Extracts from HUVEC cells Lane 4 : Extracts from HepG2 cells Lane 5 : Extracts from Jurkat cells Lane 6 : Extracts from HUVEC cells with immunising peptide at 5 µg