Recombinant
RabMAb

Anti-NDUFA8 antibody [EPR15303] (ab184952)

Overview

  • Product name
    Anti-NDUFA8 antibody [EPR15303]
    See all NDUFA8 primary antibodies
  • Description
    Rabbit monoclonal [EPR15303] to NDUFA8
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NDUFA8 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P51970

  • Positive control
    • Human fetal heart, Human fetal muscle, MCF7 and HepG2 lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab184952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50 - 1/70.
WB 1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa).

Target

  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities
    Belongs to the complex I NDUFA8 subunit family.
    Contains 2 CHCH domains.
  • Domain
    Contains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
  • Post-translational
    modifications
    May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.
  • Cellular localization
    Mitochondrion. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI 19KD antibody
    • CI PGIV antibody
    • CI-19kD antibody
    • CI-PGIV antibody
    • Complex I 19k antibody
    • Complex I 19kD antibody
    • Complex I PGIV antibody
    • Complex I PGIV subunit antibody
    • Complex I-19kD antibody
    • Complex I-PGIV antibody
    • MGC793 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 (19kD PGIV) antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 19kDa antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 antibody
    • NADH ubiquinone oxidoreductase 19 kDa subunit antibody
    • NADH ubiquinone oxidoreductase PGIV subunit antibody
    • NADH-ubiquinone oxidoreductase 19 kDa subunit antibody
    • NDUA8_HUMAN antibody
    • NDUFA 8 antibody
    • NDUFA8 antibody
    • OTTHUMP00000022034 antibody
    • PGIV antibody
    see all

Images

  • All lanes : Anti-NDUFA8 antibody [EPR15303] (ab184952) at 1/20000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal muscle lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 20 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • Western blot analysis of NDUFA8 in Human fetal heart lysate immunoprecipitated with ab184952 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal heart lysate.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab184952 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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