Overview

  • Product name
    Anti-NDUFAF1 antibody [EPR2795]
    See all NDUFAF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2795] to NDUFAF1
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NDUFAF1

  • Positive control
    • HeLa, 293T, A549 and HepG2 cell lysates. Human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
IP 1/50.
IHC-P 1/100 - 1/250.
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/1000.

Target

  • Function
    Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the CIA30 family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 65 antibody
    • CGI65 antibody
    • CIA30 antibody
    • CIA30_HUMAN antibody
    • Complex I intermediate associated protein 30 antibody
    • Complex I intermediate associated protein 30 mitochondrial antibody
    • Complex I intermediate-associated protein 30, mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody
    • NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody
    • NDUFAF 1 antibody
    • Ndufaf1 antibody
    see all

Images

  • All lanes : Anti-NDUFAF1 antibody [EPR2795] (ab92454) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 38 kDa
  • Paraffin embedded Human liver tissue labelled with ab92454 at 1/100 dilution.
  • Immunofluorescence analysis of HeLa cells labelled with ab92454 at 1/500 dilution.

References

This product has been referenced in:
  • Lindfors C  et al. Hypothalamic mitochondrial dysfunction associated with anorexia in the anx/anx mouse. Proc Natl Acad Sci U S A 108:18108-13 (2011). WB . Read more (PubMed: 22025706) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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