Overview

  • Product nameAnti-NDUFAF1 antibody [EPR2795]
    See all NDUFAF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2795] to NDUFAF1
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NDUFAF1

  • Positive control
    • HeLa, 293T, A549 and HepG2 cell lysates. Human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
IP 1/50.
IHC-P 1/100 - 1/250.
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/1000.

Target

  • FunctionChaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the CIA30 family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 65 antibody
    • CGI65 antibody
    • CIA30 antibody
    • CIA30_HUMAN antibody
    • Complex I intermediate associated protein 30 antibody
    • Complex I intermediate associated protein 30 mitochondrial antibody
    • Complex I intermediate-associated protein 30, mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody
    • NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody
    • NDUFAF 1 antibody
    • Ndufaf1 antibody
    see all

Anti-NDUFAF1 antibody [EPR2795] images

  • All lanes : Anti-NDUFAF1 antibody [EPR2795] (ab92454) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 38 kDa
  • Paraffin embedded Human liver tissue labelled with ab92454 at 1/100 dilution.
  • Immunofluorescence analysis of HeLa cells labelled with ab92454 at 1/500 dilution.

References for Anti-NDUFAF1 antibody [EPR2795] (ab92454)

This product has been referenced in:
  • Lindfors C  et al. Hypothalamic mitochondrial dysfunction associated with anorexia in the anx/anx mouse. Proc Natl Acad Sci U S A 108:18108-13 (2011). WB . Read more (PubMed: 22025706) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab92454.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"