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Product name Anti-NDUFAF1 antibody [EPR2796] See all NDUFAF1 primary antibodies
Rabbit monoclonal [EPR2796] to NDUFAF1
Tested applications Suitable for:
WB, IP, ICC/IF, IHC-P more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide from the C terminal of Human NDUFAF1
WB: HeLa, Jurkat and heart lysates;
IHC-P: liver tissue;
ICC/IF: HeLa cells.
Produced using Abcam's RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR2796
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 38 kDa).
1/100 - 1/500.
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes Is unsuitable for Flow Cyt.
Function Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
Tissue specificity Ubiquitous.
Sequence similarities Belongs to the CIA30 family.
Cellular localization Mitochondrion.
Information by UniProt
CGI 65 antibody
Anti-NDUFAF1 antibody [EPR2796] images
Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2796] (ab79826)
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling NDUFAF1 with purified 79826 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor
® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
Western blot - NDUFAF1 antibody [EPR2796] (ab79826)
All lanes : Anti-NDUFAF1 antibody [EPR2796] (ab79826) at 1/10000 dilution Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : Human heart lysate Lysates/proteins at 10 µg per lane. Secondary Goat anti Rabbit HRP-conjugated at 1/2000 dilution Predicted band size : 38 kDa Observed band size : 33 kDa ( why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NDUFAF1 antibody [EPR2796] (ab79826)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human liver tissue using 1/100 ab79826.
Immunocytochemistry/ Immunofluorescence - NDUFAF1 antibody [EPR2796] (ab79826)
Immunofluorescence analysis of HeLa cells using 1/100 ab79826.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"