Overview

  • Product nameAnti-NDUFAF1 antibody [EPR2796]
    See all NDUFAF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2796] to NDUFAF1
  • Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C terminal of Human NDUFAF1

  • Positive control
    • WB: HeLa, Jurkat and heart lysates; IHC-P: liver tissue; ICC/IF: HeLa cells.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab79826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 38 kDa).
IP 1/40.
ICC/IF 1/100 - 1/500.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionChaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the CIA30 family.
    • Cellular localizationMitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • CGI 65 antibody
      • CGI65 antibody
      • CIA30 antibody
      • CIA30_HUMAN antibody
      • Complex I intermediate associated protein 30 antibody
      • Complex I intermediate associated protein 30 mitochondrial antibody
      • Complex I intermediate-associated protein 30, mitochondrial antibody
      • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody
      • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody
      • NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody
      • NDUFAF 1 antibody
      • Ndufaf1 antibody
      see all

    Anti-NDUFAF1 antibody [EPR2796] images

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling NDUFAF1 with purified 79826 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • All lanes : Anti-NDUFAF1 antibody [EPR2796] (ab79826) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti Rabbit HRP-conjugated at 1/2000 dilution

      Predicted band size : 38 kDa
      Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human liver tissue using 1/100 ab79826.
    • Immunofluorescence analysis of HeLa cells using 1/100 ab79826.

    References for Anti-NDUFAF1 antibody [EPR2796] (ab79826)

    This product has been referenced in:
    • Miwa S  et al. Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice. Nat Commun 5:3837 (2014). WB ; Mouse . Read more (PubMed: 24815183) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"