Overview

  • Product name
    Anti-NDUFAF1 antibody [EPR2796]
    See all NDUFAF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2796] to NDUFAF1
  • Tested applications
    Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C terminal of Human NDUFAF1

  • Positive control
    • WB: HeLa, Jurkat and heart lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 38 kDa).
IP 1/40.
ICC/IF 1/100 - 1/500.
Flow Cyt 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the CIA30 family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 65 antibody
    • CGI65 antibody
    • CIA30 antibody
    • CIA30_HUMAN antibody
    • Complex I intermediate associated protein 30 antibody
    • Complex I intermediate associated protein 30 mitochondrial antibody
    • Complex I intermediate-associated protein 30, mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody
    • NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody
    • NDUFAF 1 antibody
    • Ndufaf1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling NDUFAF1 with purified 79826 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • All lanes : Anti-NDUFAF1 antibody [EPR2796] (ab79826) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Human heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti Rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size : 38 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NDUFAF1 (red) with ab79826 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human liver tissue using 1/100 ab79826.
  • Immunofluorescence analysis of HeLa cells using 1/100 ab79826.

References

This product has been referenced in:
  • Miwa S  et al. Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice. Nat Commun 5:3837 (2014). WB ; Mouse . Read more (PubMed: 24815183) »
  • Ekman M  et al. HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Lab Invest 94:557-68 (2014). Read more (PubMed: 24589856) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab79826.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up