Overview

  • Product nameAnti-NDUFB10 antibody
    See all NDUFB10 primary antibodies
  • Description
    Mouse polyclonal to NDUFB10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full-length human protein, NDUFB10 (NP_004539.1, 172 a.a).

  • Positive control
    • NDUFB10 transfected 293T cell lysate and human kidney lysate

Applications

Our Abpromise guarantee covers the use of ab70206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects bands of approximately 19 and 23 kDa (predicted molecular weight: 21 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
    • Sequence similaritiesBelongs to the complex I NDUFB10 subunit family.
    • Cellular localizationMitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 0610011B04Rik antibody
      • 22kDa antibody
      • CI PDSW antibody
      • CI-PDSW antibody
      • Complex I PDSW antibody
      • Complex I PDSW subunit antibody
      • Complex I-PDSW antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 10 22kDa antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 antibody
      • NADH ubiquinone oxidoreductase PDSW subunit antibody
      • NADH-ubiquinone oxidoreductase PDSW subunit antibody
      • NDUBA_HUMAN antibody
      • NDUFB 10 antibody
      • Ndufb10 antibody
      • PDSW antibody
      see all

    Anti-NDUFB10 antibody images

    • Anti-NDUFB10 antibody (ab70206) at 1/500 dilution + human kidneys at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 21 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-NDUFB10 antibody (ab70206) at 1/500 dilution

      Lane 1 : transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 21 kDa
      Observed band size : 19 kDa (why is the actual band size different from the predicted?)

    References for Anti-NDUFB10 antibody (ab70206)

    This product has been referenced in:
    • Hebert-Chatelain E  et al. Preservation of NADH ubiquinone-oxidoreductase activity by Src kinase-mediated phosphorylation of NDUFB10. Biochim Biophys Acta 1817:718-25 (2012). Read more (PubMed: 22321370) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab70206.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"