MitoSciences (MS107)

Anti-NDUFB4 antibody [17G3D9E12] (ab110243)

Overview

  • Product nameAnti-NDUFB4 antibody [17G3D9E12]See all NDUFB4 primary antibodies ...
  • Description
    Mouse monoclonal [17G3D9E12] to NDUFB4
  • Tested applicationsIHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    NDUFB4 from cow

  • Positive control
    • Isolated mitochondria from Human heart. HeLa cels. Cultured Human fibroblasts.

Properties

Applications

Our Abpromise guarantee covers the use of ab110243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.
ICC/IF Use a concentration of 5 - 15 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. (heat-induced antigen-retrieval improves signal).

Target

  • FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similaritiesBelongs to the complex I NDUFB4 subunit family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B15 antibody
    • CI-B15 antibody
    • Complex I B15 subunit antibody
    • Complex I-B15 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 15kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 antibody
    • NADH ubiquinone oxidoreductase B15 subunit antibody
    • NADH-ubiquinone oxidoreductase B15 subunit antibody
    • NDUB4_HUMAN antibody
    • NDUFB4 antibody
    see all

Anti-NDUFB4 antibody [17G3D9E12] images

  • All lanes : Anti-NDUFB4 antibody [17G3D9E12] (ab110243) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from cow heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg


    Predicted band size : 15 kDa
  • Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
    Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110243 at 15µg/ml, followed by a fluorescent goat-anti-mouse IgG.
  • HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunohistochemistical detection of Anti-NDUFB4 antibody [17G3D9E12] (ab110243; 1/100, incubated for 1hr) on formaldehyde fixed human cerebellum sections. Antigen retrieval step: Heat mediated. Permeabilization: No. ab110243 was used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. Non-specific background staining was not evident, and positive staining was crisp and punctate. Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit. SigmaFast DAB tablets were used for the final stage of staining.

References for Anti-NDUFB4 antibody [17G3D9E12] (ab110243)

This product has been referenced in:
  • Hämäläinen RH  et al. Tissue- and cell-type-specific manifestations of heteroplasmic mtDNA 3243A>G mutation in human induced pluripotent stem cell-derived disease model. Proc Natl Acad Sci U S A 110:E3622-30 (2013). Read more (PubMed: 24003133) »
  • Sheftel AD  et al. The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturation. Mol Biol Cell 23:1157-66 (2012). WB ; Human . Read more (PubMed: 22323289) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain - Cerebellum)
Specification Brain - Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA
Permeabilization No
Username

Ms. Philippa Hepplewhite

Verified customer

Submitted Jun 28 2012

Thank you for your enquiry,

I am sorry to confirm that this kit is no longer available, and apologise for the inconvenience.

I can confirm that we do sell the separate antibody components which you may like to consider:

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Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

Although that produ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"