The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Belongs to the complex I NDUFB7 subunit family.
Contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
Immunofluorescence analysis of, 4% paraformaldehyde-fixed, HeLa cells labelling NDUFB7 with ab188575 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
Flow cytometry analysis of 2% paraformaldehyde-fixed PC3 cells labelling NDUFB7 with ab188575 at a 1/340 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).