Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)


  • Product name
    Anti-NDUFB8 antibody [20E9DH10C12]
    See all NDUFB8 primary antibodies
  • Description
    Mouse monoclonal [20E9DH10C12] to NDUFB8
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    NDUFB8 from Cow

  • Positive control
    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart. Skeletal muscle tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab110242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-Fr Use at an assay dependent concentration.


  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities
    Belongs to the complex I NDUFB8 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASHI antibody
    • CI-ASHI antibody
    • Complex I ASHI subunit antibody
    • Complex I-ASHI antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
    • NADH-ubiquinone oxidoreductase ASHI subunit antibody
    • NDUB8_HUMAN antibody
    • NDUFB8 antibody
    see all

Anti-NDUFB8 antibody [20E9DH10C12] images

  • Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.
  • All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from cow heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from mouse heart at 10 µg

    Predicted band size : 22 kDa
    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

References for Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)

This product has been referenced in:
  • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . Read more (PubMed: 27545886) »
  • Kauppila JH  et al. A Phenotype-Driven Approach to Generate Mouse Models with Pathogenic mtDNA Mutations Causing Mitochondrial Disease. Cell Rep 16:2980-90 (2016). Read more (PubMed: 27626666) »

See all 47 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (Human brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
10 µg
Human brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Sandrine Wauters

Verified customer

Submitted Apr 01 2016

Western blot
Mouse Cell lysate - other (Mouse mitos from liver (A) and muscle (B))
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-12%)
Loading amount
10 µg
Mouse mitos from liver (A) and muscle (B)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 29 2015

Thank you for contacting us.

The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

I hope this information is helpful...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Brain- Cerebellum)
Brain- Cerebellum
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA

Ms. Philippa Hepplewhite

Verified customer

Submitted Jun 28 2012

Thank you for your call earlier.

I now had a look at the homology between the immunogens of the antibodies included in the MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413) and the respective proteins in Drosophila.
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Thank you for this additional information. I can confirm that ab110413 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail) contains 5 antibodies. The datasheets and more information about these antibodies can be found when following...

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