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Rabbit polyclonal to NDUFC2
Predicted to work with:
Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee, African green monkey
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (IARRNPEPLR FLPDEARSLP PPKLTDPRLL YIGFLGYCSG LIDNLIRRRP) of human NDUFC2 (NP_004540).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Belongs to the complex I NDUFC2 subunit family.
Mitochondrion inner membrane.
Information by UniProt
Complex I B14.5b antibody
Western blot - NDUFC2 antibody (ab85359)
Anti-NDUFC2 antibody (ab85359) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
10 kDa (
why is the actual band size different from the predicted?
Additional bands at:
22 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"