Overview

  • Product name
  • Description
    Rabbit polyclonal to NDUFC2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee, African green monkey
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (IARRNPEPLR FLPDEARSLP PPKLTDPRLL YIGFLGYCSG LIDNLIRRRP) of human NDUFC2 (NP_004540).

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities
    Belongs to the complex I NDUFC2 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B14.5b antibody
    • CI-B14.5b antibody
    • Complex I B14.5b antibody
    • Complex I subunit B14.5b antibody
    • Complex I-B14.5b antibody
    • HLC-1 antibody
    • HLC1 antibody
    • Human lung cancer oncogene 1 protein antibody
    • NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 subunit C2 antibody
    • NADH dehydrogenase ubiquinone 1 subunit C2 antibody
    • NADH ubiquinone oxidoreductase subunit B14.5b antibody
    • NADH-ubiquinone oxidoreductase subunit B14.5b antibody
    • NADHDH2 antibody
    • NDUC2_HUMAN antibody
    • NDUFC2 antibody
    see all

Images

  • Anti-NDUFC2 antibody (ab85359) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 14 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

References

ab85359 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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